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Source / Quelle: https://www.mdpi.com/1420-3049/31/14/2498 Abstract The aim of the study was to determine the effect of cannabidiol extract (CBD) on the activity of the proteolytic system and the concentrations of total proteins on the cuticular surface of worker bees. Marked one-day-old workers were introduced into established colonies, which were divided into three groups (two hives each): CSy (CBD in sugar syrup), CSt (CBD-soaked strip), and C (control, pure syrup). Every seven days, the bioactive cuticular layer was washed off from the marked bees for biochemical analysis. Total protein concentrations increased with worker age in C, CSy, and CSt, and were highest from day 7 in group C. Protease activities increased until day 21/28 of worker life in all groups. From day 21 for acidic proteases, day 28 for neutral proteases, and day 14 for alkaline proteases, the activities were highest in CSy. The activities of neutral and alkaline protease inhibitors decreased with bee age and were

Abstract 4474: Cannabidiol enhances etoposide efficacy in non-small cell lung cancer by engaging a p53-mTOR-TFEB lysosomal death program. Source / Quelle: https://aacrjournals.org/cancerres/article/86/7_Supplement/4474/777680 Abstract The search for more effective and safer cancer therapies has intensified interest in combination regimens that repurpose well-established agents. Here, we investigated whether cannabidiol (CBD), a cannabis-derived compound clinically used for pediatric epilepsy, can potentiate the anticancer activity of etoposide in non-small cell lung cancer (NSCLC). Among multiple chemotherapeutics tested, etoposide displayed the most pronounced reduction in NSCLC cell viability when combined with CBD, indicating a robust synergistic interaction. To elucidate the underlying mechanisms, we performed comprehensive transcriptomic and proteomic profiling, revealing that the CBD-etoposide combination up-regulated gene programs associated with autophagic cell death while conc

Harnessing Trichoderma Species for Sustainable Biocontrol: Mechanisms, Formulation Strategies, Commercialization, and Field Applications Source / Quelle: https://www.mdpi.com/2223-7747/15/15/2260 Abstract Trichoderma species are widely investigated and commercially applied as eco-friendly biocontrol agents in sustainable agriculture. These filamentous fungi protect plants through multiple complementary mechanisms, including mycoparasitism, antibiosis, competition for nutrients and ecological niches, and induction of systemic resistance in host plants. These activities are mediated by a diverse array of secondary metabolites, hydrolytic enzymes, and signaling pathways that collectively suppress pathogens and enhance plant health. Beyond disease control, selected Trichoderma strains promote plant growth by improving nutrient acquisition, modulating phytohormone signaling, and increasing tolerance to abiotic stresses. This review summarizes recent advances in the mechanisms underlying Tri

The Effect of Cannabidiol on Cancer-Pathway Genes in Doxorubicin-Sensitive and Resistant Breast Cancer Cells Source / Quelle: https://www.mdpi.com/1424-8247/19/4/615 Abstract Purpose: Cannabidiol (CBD) is a primary bioactive, non-intoxicating cannabinoid found in the cannabis plant. Studies have shown that CBD causes anticancer activity by inhibiting the expression of growth factors and inducing apoptosis, leading to cell cycle arrest. In this study, we aimed to determine how CBD influences the expression of genes that affect cancer pathways in doxorubicin-sensitive (MCF-7) and doxorubicin-resistant (MCF-7/Adr) breast cancer cells. Materials and Methods: IC50 concentrations of CBD in MCF-7 and MCF-7/Adr cell lines were determined by the MTT cell cytotoxicity assay. RNA isolation and subsequent cDNA synthesis were performed for qPCR experiments with the determined IC50 values. The effects of CBD on the cell cycle and apoptosis were studied using flow cytometry. IC50 values of CBD were d

Cannabinoids Characterization and the Anti-cancer Activity of Cannabis sativa Stem Extracts against Colorectal Cancer Cell Source / Quelle: https://lafiascijournals.org.ng/index.php/ljsir/article/view/808 Department of Chemistry, Federal University of Lafia, PMB 146, Nasarawa State, Nigeria Abstract Cannabis sativa is a plant with a growing interests in research, and this is because of it therapeutic value associated with its active constituents such as cannabinoids and the terpenes. In this study, we characterized some isolated cannabinoids from the stems of cannabis plant using a JEOL Delta GX 400 MHz FT nuclear magnetic resonance instrument, and also evaluate the anti-cancer activity of the extracts against colorectal cancer cell. The cell viability was assessed using AlamarBlue and readings were recorded in term of fluorescence values using a microplate reader. Two cannabinoids were isolated (cannabinol and tetrahydrocannabinolic acid) from the stems. HCT 116 cancerous cell was use

Source / Quelle: https://link.springer.com/article/10.1007/s00203-026-04886-7 Abstract Avian infectious bronchitis virus (IBV), a gamma coronavirus, remains the predominant pathogen affecting poultry worldwide, posing a significant threat to the poultry industry. Cannabidiol (CBD), the primary non-psychoactive constituent of Cannabis sativa, has demonstrated diverse pharmacological properties. This study investigated the inhibitory effects of CBD against IBV infection both in vitro and in vivo. Chicken embryo kidney (CEK) cells infected with IBV were treated with CBD at various concentrations (2–20 µM). Our results demonstrated that CBD significantly suppressed IBV replication in a dose-dependent manner without affecting cell viability. Furthermore, in SPF chickens, CBD treatment markedly alleviated clinical symptoms, reduced tracheal and renal histopathological lesions, and significantly decreased viral loads in tissues. Transcriptomic analysis of tracheal tissue suggested that CBD ma